platebound α-cd3 antibody Search Results


cd3 epsilon antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 epsilon antibody
Cd3 Epsilon Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd3 epsilon antibody/product/NSJ Bioreagents
Average 99 stars, based on 1 article reviews
cd3 epsilon antibody - by Bioz Stars, 2026-05
99/100 stars
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soluble α cd28  (Bio X Cell)
96
Bio X Cell soluble α cd28
(A) Dose dependent inhibition of GAPDH and hexokinase activities in a lymph nodes single cell suspension by 3-BrPa. (B) Effect of 3-BrPa on T cell proliferation. Lymph node cells were stimulated with α-CD3 (1 μg/mL) and <t>α-CD28</t> (10 μg/mL). At 24 hours, a time when glycolysis is engaged, but no cell divisions have occurred, 3-BrPa was added to a final concentration of 10 μM, and subsequent divisions were assessed after 48 additional hours. Proliferation of CD8+ T cells is shown. Gated on live, singlet CD8+ cells. (C) Effect of increasing concentrations of 3-BrPa on TNFα and IL-2 production in T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL). Gated on live, CD11b− CD19− TCRβ+ CD4+ or CD8+. (D) Effect of 3-BrPa on CD69 induction on T cells after 6 hours of activation with α-CD3 (1 μg/mL) α-CD28 (2 μg/mL). Gated on live, singlet, CD11b− B220− CD4+ (E) Effect of 3-BrPa on phosphorylation of ERK in TCRβ+ CD4+ T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL) for 60min. Gated on singlet, TCRβ+ CD4+. The same results were observed in CD8+ T cells
Soluble α Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/soluble α cd28/product/Bio X Cell
Average 96 stars, based on 1 article reviews
soluble α cd28 - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


(A) Dose dependent inhibition of GAPDH and hexokinase activities in a lymph nodes single cell suspension by 3-BrPa. (B) Effect of 3-BrPa on T cell proliferation. Lymph node cells were stimulated with α-CD3 (1 μg/mL) and α-CD28 (10 μg/mL). At 24 hours, a time when glycolysis is engaged, but no cell divisions have occurred, 3-BrPa was added to a final concentration of 10 μM, and subsequent divisions were assessed after 48 additional hours. Proliferation of CD8+ T cells is shown. Gated on live, singlet CD8+ cells. (C) Effect of increasing concentrations of 3-BrPa on TNFα and IL-2 production in T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL). Gated on live, CD11b− CD19− TCRβ+ CD4+ or CD8+. (D) Effect of 3-BrPa on CD69 induction on T cells after 6 hours of activation with α-CD3 (1 μg/mL) α-CD28 (2 μg/mL). Gated on live, singlet, CD11b− B220− CD4+ (E) Effect of 3-BrPa on phosphorylation of ERK in TCRβ+ CD4+ T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL) for 60min. Gated on singlet, TCRβ+ CD4+. The same results were observed in CD8+ T cells

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Lineage-specific metabolic properties and vulnerabilities of T cells in the demyelinating central nervous system

doi: 10.4049/jimmunol.1600825

Figure Lengend Snippet: (A) Dose dependent inhibition of GAPDH and hexokinase activities in a lymph nodes single cell suspension by 3-BrPa. (B) Effect of 3-BrPa on T cell proliferation. Lymph node cells were stimulated with α-CD3 (1 μg/mL) and α-CD28 (10 μg/mL). At 24 hours, a time when glycolysis is engaged, but no cell divisions have occurred, 3-BrPa was added to a final concentration of 10 μM, and subsequent divisions were assessed after 48 additional hours. Proliferation of CD8+ T cells is shown. Gated on live, singlet CD8+ cells. (C) Effect of increasing concentrations of 3-BrPa on TNFα and IL-2 production in T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL). Gated on live, CD11b− CD19− TCRβ+ CD4+ or CD8+. (D) Effect of 3-BrPa on CD69 induction on T cells after 6 hours of activation with α-CD3 (1 μg/mL) α-CD28 (2 μg/mL). Gated on live, singlet, CD11b− B220− CD4+ (E) Effect of 3-BrPa on phosphorylation of ERK in TCRβ+ CD4+ T cells stimulated with α-CD3 (1 μg/mL) and α-CD28 (2 μg/mL) for 60min. Gated on singlet, TCRβ+ CD4+. The same results were observed in CD8+ T cells

Article Snippet: To assess the effects of 3-BrPa on T cell proliferation, T cells were isolated from spleens and lymph nodes of C57BL/6 mice using the R&D CD3 + T cell enrichment Column (MTCC-10), stained with 20 μM Cell Proliferation Dye eFluor450 (eBioscience #65-0842-85), and stimulated to divide in complete RPMI with platebound α-CD3 (BioXcell #BE0001-1) (1 μg/mL) and soluble α-CD28 (BioXcell #BE0015-5) (10 μg/mL) antibodies for 72hrs.

Techniques: Inhibition, Suspension, Concentration Assay, Activation Assay, Phospho-proteomics

ELISA (A) and qRT-PCR (B) for IFN-γ and IL-17A production by in vitro derived Th1 and Th17 cells stimulated with α-CD3 (2 μg/mL) and α-CD28 (2 μg/mL) in the presence of 3-BrPa (10 μM) for 20 hours; (C) flow cytometry for IFN-γ and IL-17A production by in vitro derived Th1 and Th17 cells stimulated as above for 6 hours in the presence of Brefeldin A. Gated on live, singlet, TCRβ+, CD4+ (A–C) are N=1 experiment with n=1–2 mice per group

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Lineage-specific metabolic properties and vulnerabilities of T cells in the demyelinating central nervous system

doi: 10.4049/jimmunol.1600825

Figure Lengend Snippet: ELISA (A) and qRT-PCR (B) for IFN-γ and IL-17A production by in vitro derived Th1 and Th17 cells stimulated with α-CD3 (2 μg/mL) and α-CD28 (2 μg/mL) in the presence of 3-BrPa (10 μM) for 20 hours; (C) flow cytometry for IFN-γ and IL-17A production by in vitro derived Th1 and Th17 cells stimulated as above for 6 hours in the presence of Brefeldin A. Gated on live, singlet, TCRβ+, CD4+ (A–C) are N=1 experiment with n=1–2 mice per group

Article Snippet: To assess the effects of 3-BrPa on T cell proliferation, T cells were isolated from spleens and lymph nodes of C57BL/6 mice using the R&D CD3 + T cell enrichment Column (MTCC-10), stained with 20 μM Cell Proliferation Dye eFluor450 (eBioscience #65-0842-85), and stimulated to divide in complete RPMI with platebound α-CD3 (BioXcell #BE0001-1) (1 μg/mL) and soluble α-CD28 (BioXcell #BE0015-5) (10 μg/mL) antibodies for 72hrs.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, In Vitro, Derivative Assay, Flow Cytometry